Journal: Stem Cell Reports

Article Title: Selective elimination of pluripotent stem cells by PIKfyve specific inhibitors

doi: 10.1016/j.stemcr.2021.12.013

Figure Lengend Snippet: WX8 induced programmed cell death selectively only in human pluripotent stem cells (A) Fibroblasts and ECCs were seeded at densities such that cells cultured in the absence of WX8 were not confluent at the end of the experiment. Cells were treated with various concentrations of WX8 for 4 days, stained with annexin-V and propidium iodide (PI) without prior permeabilization using an Annexin V-FITC Apoptosis Detection Kit, and then analyzed in a Becton Dickinson LSR-II using FACS Diva software. (B) SEM is indicated for average of three independent assays. Cells cultured in the presence of vehicle (0 μM WX8) were defined as 100% viable cells (Q3 • ▪) and 0% dead cells (Q1+Q2 ◯ □) so that only changes due to WX8 were represented. Time 0 h was plotted as 0.1 h to generate a logarithmic scale. Similar results were obtained with ESCs and iPSCs ( Figure S5 ).

Article Snippet: Fluorescein isothiocyanate (FITC) and propidium iodide (PI) fluorescence were detected with BD LSR-II using FACS Diva software (Becton Dickinson).

Techniques: Cell Culture, Staining, Software